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nucleofection kit v (Lonza)

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Lonza nucleofection kit v
Nucleofection Kit V, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nucleofection+v+kit/4d+nucleofector/pm39826696-351-21-25
Average 90 stars, based on 1 article reviews

nucleofection kit v - by Bioz Stars, 2026-11

90/100 stars

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Knock-Out:

Article Title: The septin cytoskeleton regulates natural killer cell lytic granule release.
Article Snippet: .. For knock-out of each gene, a complex was formed by combining gRNA/tracrRNA complex of each gRNA at a 1:1 ratio with 30 μg of Cas9 enzyme at room temperature for 10 min. NKL cells were nucleofected with the complex using the CM-137 program on a Lonza 4D nucleofector and Nucleofection V kit (Lonza). ..

Electroporation:

Article Title: Vasodilator Stimulated Phosphoprotein (VASP) Regulates Natural Killer Cell Lytic Granule Convergence
Article Snippet: .. Nucleofection of siRNA oligos into NKL, primary human NK cells and KHYG-1 was done using the standard protocol and Nucleofection V kit (Lonza) and an Amaxa Nucleofector I as previously described ( 39 , 40 ). ..

other:

Article Title: The septin cytoskeleton regulates natural killer cell lytic granule release
Article Snippet: siRNAs used included a control siRNA (siCtrl: 5′-UUC UCC GAA CGU GUC ACG U-3′), two siRNAs against septin 2 (1–5′-GAU CCA AUC AGU UGA UUG AAG CCA A-3′ and 2–5′-UGU CCA GAU UGA GGC UUC AAC UGU U-3′), and two siRNAs against septin 7 (1–5′-GCU UAU GGU AGU GGG UGA AUC UGG A-3′ and 2–5′-GCU GAG GAG AGG AGC GUC AAC AGC A-3′; Thermo Fisher Scientific).

Plasmid Preparation:

Article Title: An engineering strategy to target activated EGFR with CAR T cells
Article Snippet: The co-transfected CRISPR/Cas9 ribonucleoprotein complex (RNP) consisted of Alt-R crRNA, tracrRNA, and Cas9/D10A nickase V3 recombinant protein (all from IDT). .. 10 6 Jurkat E6.1 cells were electroporated using an Amaxa Nucleofector 2b (program X-005) and Nucleofection V Kit (Lonza), 5 μg circular donor plasmid and 250 pmol RNP. .. 10 6 Jurkat E6.1 cells were electroporated using an Amaxa Nucleofector 2b (program X-005) and Nucleofection V Kit (Lonza), 5 μg circular donor plasmid and 250 pmol RNP.